Part:BBa_K4074049:Design

pSB_THRC_GIBSON

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 1187
  Illegal suffix found at 3666
  Illegal EcoRI site found at 1342
  Illegal EcoRI site found at 2367
  Illegal EcoRI site found at 3448
  Illegal XbaI site found at 2340
  Illegal PstI site found at 2328
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 1342
  Illegal EcoRI site found at 2367
  Illegal EcoRI site found at 3448
  Illegal SpeI site found at 3667
  Illegal PstI site found at 2328
  Illegal PstI site found at 3681
  Illegal NotI site found at 1193
  Illegal NotI site found at 3655
  Illegal NotI site found at 3674
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 1342
  Illegal EcoRI site found at 2367
  Illegal EcoRI site found at 3448
  Illegal BamHI site found at 2346
  Illegal XhoI site found at 171
  Illegal XhoI site found at 1063
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 1187
  Illegal suffix found at 3667
  Illegal EcoRI site found at 1342
  Illegal EcoRI site found at 2367
  Illegal EcoRI site found at 3448
  Illegal XbaI site found at 2340
  Illegal PstI site found at 2328
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 1187
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1342
  Illegal EcoRI site found at 2367
  Illegal EcoRI site found at 3448
  Illegal XbaI site found at 1202
  Illegal XbaI site found at 2340
  Illegal SpeI site found at 3667
  Illegal PstI site found at 2328
  Illegal PstI site found at 3681
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

Design Notes

-

Source

pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab